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Protein-Based Technology 2

BOT106

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BOT106 - Protein-Based Technology 2

Prerequisites:

BOT100

In this course students learn about the production and expression of recombinant proteins. Topic include; gene amplification and sub-cloning into expression vectors, expression and purification by His-Ni affinity chromatography; qualitative and semi-quantitative analysis by Western blot, ELISA and radio-immunoassays (RIA); examples of applications in different biotechnology sectors; laboratory practices.


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