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BIC373 - Biochemistry

This course extends the student's knowledge regarding theory and techniques applied in the field of biotechnology by exploring the biochemical reactions that govern life and laboratory-based conclusions. Students will recognize how the molecular environment surrounding biological macromolecules dictates organization and interactions while considering the implications of thermodynamics to the control. Successfully conceptualizing the molecular basis of the interactions that occur between functional groups of various macromolecules in aqueous environments and how these interactions affect functional outcomes in living systems or biotechniques is a critical milestone in a biotechnologist's learning.

Emphasis will be placed on the structure, function and metabolism of amino acids, proteins and DNA and how these molecules produce the work required for the lifecycle of a cell. Production of nucleic acids and lipids from metabolic activities, genetic control, energy transfer, membrane dynamics and cell signaling cascades including primary, secondary and lipid messengers involved in metabolism and regulation of gene expression will be discussed with regards to the synthesis of functional proteins.

Throughout the course, the association of academic and Industrial professional environments will be discussed. The molecular mechanisms underlying various laboratory techniques currently used to investigate amino acids, protein, nucleic acids, and lipids will be presented. For example, the application of phase separations will be explored through current macromolecular research initiatives in biotechnology.

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BIT373 - Biotechniques

This laboratory course is an introduction to the fundamental practices of biological macromolecular biochemistry as would be used in a modern biotechnology laboratory. Topics include the requirement of buffers for biotechnique use. Online exercises and activities focus on the theory regarding the isolation and analysis of proteins and biologically relevant small molecules. Application of various forms of chromatography, dialysis and membrane filtration to the separation and analysis of these molecules are discussed. The use of assays to detect and quantify biologically relevant molecules will be explored with topics such as immunoblot analysis, enzyme use and assays for biotechnology and the detection and quantification of biological molecules such as proteins and nucleic acids.

The combination of multi-step analyses for inference of conclusions in the biotechniques lab will be presented using the results from multiple practical techniques of biotechnological assays. Practical techniques include stock buffer and working solution preparation, accurate and precise techniques, colorimetric protein assays, column chromatography, and electrophoretic separation of macromolecules. Students calculate mathematical parameters of interest through the processes studied and use computer software to present graphical and tabular results and introductory statistical analyses in experimental error. A hands-on approach and the development of practical laboratory skills, time management for efficient laboratory work, and generation of accurate and precise data are emphasized.

Biochemistry (BIC 373) is a mandatory co-requisite course for this subject; the theory behind the techniques used in lab will be discussed both in the lab (BIT 373) and in the lecture (BIC373) co-requisites.

 

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CHO353 - Chemistry - Organic

A number of classes of organic compounds will be studied from the point of view of their structure, nomenclature, synthesis, reactions and applications. In the laboratory portion of the subject, separation techniques and some simple identification techniques will be learned.

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MBG353 - Microbiology

An introductory course which examines the structure and physiology of microorganisms. Laboratory exercises introduce basic microbiological techniques including staining and microscopy, media preparation and culture techniques, quantitation of microbial populations, the examination of physical and chemical factors which affect the growth of microbes, and methods used to isolate and identify common bacteria.

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SES391 - Effective Technical Writing

This subject focuses on technical writing skills for biochemistry students. After an introduction to basic communication elements (audience analysis, technical style, routine correspondence), students will write summaries, standard operating procedures, and informal reports; use visual aids; develop oral presentation strategies; and update career packages. Emphasis throughout will be placed on effective writing strategies and editing techniques. .
Prerequisite: EAC150 or equivalent

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