BIC633 - Biochemistry for Analytical Chemistry

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Last revision date 2017-01-30 00:23:40.523
Last review date 2017-04-03 00:15:32.681

Subject Title
Biochemistry for Analytical Chemistry

Subject Description
This course is designed to introduce chemistry majors to the basic, current principles and techniques commonly used in laboratories that may employ graduates of the CHY programme. The course will focus on several general techniques and will relate biochemical/microbiological procedures to specific analytical chemistry methods. This course will also explore basic microbiological techniques since there is a great trend towards using microbes in traditional chemical fields including Environmental Remdiation. Further, many inorganic metals and polymers are compriomised by microbiological invasion. Consequently, a rudimentary understanding of microbes and simple biochemistry will augment a chemists relevance in the workplace. The course consists of both lecture and laboratory components. Graduation from the course will require successful completion of both components

Credit Status
One credit towards the following Technology Diploma Program: CHY, both co-op and non-coop programs

Learning Outcomes
Upon successful completion of this subject the student will be able to:

Upon successful completion of this subject the student will:
1)            Have demonstrated an understanding of the basic techniques utilized in Microbiology and biotechnology.
2)            Have demonstrated acquisition of the theories presented in class such as basic microbiology, protein, lipid, carbohydrate, and DNA chemistry.
3)            Will show an appreciation of where biotechnology will progress in the next few years and how these topics relate to Chemical Engineering.
More specifically:
1. Understand the relationship of macromolecules to biological systems and the molecular network involved in metabolism
2. Understand the importance of buffers in cellular and laboratory environments.
3. Understand how to prepare a buffer.
4. Know the difference between theoretical (Henderson-Hasselbach) and practical buffer preparation and calculations.
5. Understand the effects of the properties of water.
6. Understand solubility and polarity and how they affect isolation of molecules.
7. Understand how commercial pharmaceuticals affect organisms at a biochemical level.
8. Understand proper graphing techniques and the potential errors in improper graphing.
9. Understand the purpose and set-up of various assay methods.
10. Use graphical techniques to determine Km and Vmax.
11. Understand the importance of Km and Vmax.
12. Know the theoretical basis for the Michaelis-Menton equation of enzyme kinetics.
13. Understand electrophoretic techniques and be able to utilize their results (agarose and acrylamide).
14. Understand the theory of column chromatography (ion exchange, affinity, and MW exclusion).
15. Read titration curves and use this information to determine molecular constituents of titratable molecules (amino acids and proteins).
16. Understand isoelectric focusing.
17. Relate pI and pKa to isolation techniques.
18. Understand the forces that form the tertiary and quaternary structure of proteins.
19. Understand the how heat, salt, pH, and reducing agents affect protein structure and function.
20. Know various post-transcriptional and post-translational modifications of macromolecules (ie. phosphorylations).
21. Understand the role of secondary structures in tertiary protein formation.
22. Understand protein spectrophotometric techniques and their strengths and limitations.
23. Identify chemotherapeutic sites in nucleotide synthesis.
23. Understand Sterile procedures and aseptic techniques
24. Culture bacteria and other organisms in the lab.
25. Familiar with the various techniques required to identify microorganisms such as, but not limited to, biochemical test, Differential media use, and PCR                .
26. Understand how bacteria grow and divide both in the lab and in their natural habitat.
27. Understand how bacteria can be used in placeof traditional chemical treatments like those used in Environmental Remediation of oil spills and removing heavy metals from mining run-offs.

Essential Employability Skills
Communicate clearly, concisely and correctly in the written, spoken and visual form that fulfils the purpose and meets the needs of the audience.

Respond to written, spoken, or visual messages in a manner that ensures effective communication.

Execute mathematical operations accurately.

Apply a systematic approach to solve problems.

Use a variety of thinking skills to anticipate and solve problems.

Locate, select, organize, and document information using appropriate technology and information systems.

Analyze, evaluate, and apply relevant information from a variety of sources.

Interact with others in groups or teams in ways that contribute to effective working relationships and the achievement of goals.

Manage the use of time and other resources to complete projects.

Take responsibility for one's own actions, decisions, and consequences.

Cheating and Plagiarism
Each student should be aware of the College's policy regarding Cheating and Plagiarism. Seneca's Academic Policy will be strictly enforced.

To support academic honesty at Seneca College, all work submitted by students may be reviewed for authenticity and originality, utilizing software tools and third party services. Please visit the Academic Honesty site on for further information regarding cheating and plagiarism policies and procedures.

All students and employees have the right to study and work in an environment that is free from discrimination and/or harassment. Language or activities that defeat this objective violate the College Policy on Discrimination/Harassment and shall not be tolerated. Information and assistance are available from the Student Conduct Office at

Accommodation for Students with Disabilities
The College will provide reasonable accommodation to students with disabilities in order to promote academic success. If you require accommodation, contact the Counselling and Disabilities Services Office at ext. 22900 to initiate the process for documenting, assessing and implementing your individual accommodation needs.