BOT102 - Analytical Chemistry

Outline info
Last revision date 2018-07-20 11:36:16.52
Last review date 2018-07-20 11:36:23.287

Subject Title
Analytical Chemistry

Subject Description
Analytical Chemistry serves as one of the foundations for ABCT program. Many industries, such as chemical, pharmaceutical, biotech and food rely heavily on chemical analysis in both quality control and research and development areas. Environmental monitoring is also strongly based on the basic analytical concepts developed in this course. It is important that the student master the basics of this subject in order to perform more advanced analytical techniques such as chromatographic and spectroscopic analyses. The chemistry involved in addition to knowledge of basic laboratory methods and data handling, calculation and presentation is central to a number of laboratory areas. In this course, students will familiarize them with sample preparation, method selection, techniques, calculations and data handling. This course also introduces the chemical principles behind gravimetric and volumetric methods of analysis. Problem-solving is strongly emphasized. The theory portion of the course will cover all the major theories in analytical chemistry. The laboratory portion of the course emphasizes good laboratory technique and practices.  Accuracy and precision of analytical results as well as documentation and presentation of laboratory results is evaluated.

Credit Status
One credit towards the Applied BioChem Techniques (ABCT) Certificate Program (APPLIED BIOTECHNOLOGY).

Learning Outcomes
Upon successful completion of this subject the student will be able to:

  1. Perform solution calculations and accurately prepare solutions using various concentration units (M, N, ppm, ppb, %w/w, %w/v, %v/v, etc.) and dilutions
  2. Prepare samples and ensure they are representative of the intended sampling population.
  3. Perform stoichiometric calculations to solve chemical analysis problems both in theory and in practice in the laboratory.
  4. Use basic statistical methods and apply them to analytical data:  (mean, median, standard deviation, simple statistical tests, linear regression using excel).
  5. Describe the theory behind gravimetric analyses and precipitation titrations (solubility product constants, calculating solubilities using Ksp, the use of indicators in precipitation titrations).
  6. Describe and explain acid/base chemistry as it applies to analytical chemical applications.  (Acid/base equilibria, weak and strong acids, acid/base titrations, indicators).
  7. Follow scientific procedures and carry out laboratory operations:  Use standard laboratory equipment in the performance of analytical procedures (analytical balances, pH meters, spectrophotometers).
  8. Present scientific information in a professional and concise report:  Interpret and report quantitative analysis results in the required format. Compile, organize prepare and present scientific and technical data and results using current software (word processing, excel spreadsheets, excel graphs) to express and manipulate data.
  9. Follow proper environmental and health and safety protocols: Handle and dispose of chemicals safely in the laboratory as indicated in departmental safety protocols. Describe, demonstrate and follow the basic protocol of GLP in the laboratory.
  10. Prepare samples and follow analytical methodology.
  11. Explain and describe the basic theory and terminology of analytical chemistry.

Cheating and Plagiarism
Each student should be aware of the College's policy regarding Cheating and Plagiarism. Seneca's Academic Policy will be strictly enforced.

To support academic honesty at Seneca College, all work submitted by students may be reviewed for authenticity and originality, utilizing software tools and third party services. Please visit the Academic Honesty site on for further information regarding cheating and plagiarism policies and procedures.

All students and employees have the right to study and work in an environment that is free from discrimination and/or harassment. Language or activities that defeat this objective violate the College Policy on Discrimination/Harassment and shall not be tolerated. Information and assistance are available from the Student Conduct Office at

Accommodation for Students with Disabilities
The College will provide reasonable accommodation to students with disabilities in order to promote academic success. If you require accommodation, contact the Counselling and Disabilities Services Office at ext. 22900 to initiate the process for documenting, assessing and implementing your individual accommodation needs.


Topic Outline
Week 1: lecture based on prescribed textbook chapter 1: making measurements: introduction, record keeping, date handling, method development. Laboratory practice introduction; acid base titration. Laboratory: basic laboratory operation (refer to companying laboratory manual); use of balances in analytical chemistry, quantitative transfers, use of a burettes and pipettes; determination of HCl concentration by simple acid-base titration.

Week 2:  lecture based on textbook chapter 2: sample preparation, extraction and analysis by chromatography. Laboratory: determination of Ni and Zn by Ion exchange chromatography

Week 3: lecture: based on chapter 3: classic methods; gravimetry, titration, coulometry. Laboratory: determination of calcium by EDTA titration.

Week 4: lecture: Chapter 4: molecular spectroscopy; EM, Electromagnetic, UV/Vis, Beer-lambert law, fluorescence. Laboratory: spectroscopic analysis of a mixture: caffeine and benzoic acid, spectrophotometric determination of glucose/manganese.

Week 5: lecture: Chapter 5: acid base equilibria; introduction, kw calculation. Mid-term exam: covering lectures weeks 1-4. Laboratory: determination of acetic acid in vinegar by acid/base titration.

Week 6: lecture: Chapter 6: buffer system; titration curve, calculations. Laboratory: determination of carbonate and bicarbonante in a mixture by acid-base titration.

Week 7: lecture: Chapter 7: complexation and precipitation; terminology, calculations. Laboratory: determination of chloride by precipitation titration.

Week 8: lecture: Chapter 8: electroanalytical chemistry; introduction, electro-chemistry methods. Laboratory: analysis of fat content from French fries by Soxhlet extraction.

Week 9: lecture: Chapter 9: atomic spectrometry; AAS, AES, MS. Laboratory: HPLC analysis of caffeine-benzoic acid mixture.

Week 10: lecture: Chapter 10: analytical separation. Final exam. Laboratory: analysis of samples using GC-MS column Agilent HP5MS.

Mode of Instruction
Lectures, Laboratory, Tutorials, Assignment Presentations, Discussions

Prescribed Texts

  • Basics of Analytical Chemistry and Chemical Equilibria by Brian M. Tissue, John Wiley & Sons, ISBN: 978-0-470-59208-3. 426 pages, July 2013
  • Analytical Chemistry laboratory manual prepared by Seneca College Faculty

Reference Material
Prescribed text books, laboratory experiment notes in form of procedures and reports

Required Supplies
Laboratory coat, safety glasses

Promotion Policy

Grading Policy
A+ 90%  to  100%
A 80%  to  89%
B+ 75%  to  79%
B 70%  to  74%
C+ 65%  to  69%
C 60%  to  64%
D+ 55%  to  59%
D 50%  to  54%
F 0%    to  49% (Not a Pass)
EXC Excellent
SAT Satisfactory
UNSAT Unsatisfactory

For further information, see a copy of the Academic Policy, available online ( or at Seneca's Registrar's Offices.

Modes of Evaluation

  • Assignments are due at the beginning of the class on which they are due.
  • A late penalty of 10% per day is assessed for late assignments, including those not handed in at the beginning of class when due.
  • Material will not be accepted after one week following the due date and/or when the marked material is returned to students, whichever comes first.
  • Assignments are to be prepared by computer.

Absenteeism and Exams/Quizzes
  • Students should be aware that absenteeism almost guarantees an inability to achieve satisfactory grades.
  • Students who are absent for an examination due to an emergency (e.g., motor vehicle accident, hospitalization or death in the family) may provide official documentation within five days of the missed exam and be provided a deferred exam at a later date.  Official documentation includes a death notice or an original doctor’s certificate identifying the date, length of time expected absence and the specific reason for the absence.  Examinations missed without official documentation and approval result in a grade of zero.
  • There are no deferred options for missed quizzes.  The value of missed quizzes, at the discretion of the Faculty, will be added to other evaluation components.

English Proficiency
  • All written work should demonstrate the following characteristics for clarity and conciseness:
-writing is consistent with the rules of English grammar
-spelling and punctuation are correct
-sentences are structured correctly
-main ideas are supported with specific, relevant examples and reasons
-work flows logically through supporting statements/paragraphs
-work is arranged in correct format (e.g., as a report, essay)
-up to 10% of the final grade may be deducted on all work if the above English competencies are not met.

Format for Assignments
  • Students must use the standard, APA style for quoting sources.   Help is available at:

Laboratory Attendance
The laboratory component is essential and therefore it is strongly recommended that you attend all labs.  Any missed labs must be supported with a legal document with three days of the lab.  Any student who fails to attend more than 2 laboratory classes for a 10 week subject will not pass the subject.    

Laboratory Safety
Students are required to review and understand the safety procedures and guidelines outlined on the first class and sign the sheet to this effect before beginning work in the laboratory.  Students must also wear a lab coat and safety glasses when conducting experiments.
A student who arrives without the proper safety equipment will not be permitted to participant in the lab but will be asked to leave the class.  The student will receive no grade for the lab missed.

Grading is based on the following marking scheme:
Mid-term and final exams each is worth 15%; each lab is worth 7%. Absent exams generally cannot be made up; lab mark will be pro-rated if two or few labs are missed with reasonable excuses (doctor’s notes etc.). No one shall miss more than two labs.


Other Evaluation Considerations
The student is expected to comply with the Safety Rules for working in the laboratory, sign the safety contract, and WILL NOT UNDER ANY CIRCUMSTANCES WEAR CONTACT LENSES in the laboratory. The student will know where all safety equipment is located in the laboratory and will be familiar with WHIMIS concepts and signage.


Approved by: Denis Gravelle