BOT105 - Protein-Based Technology 1

Outline info
Last revision date 2018-07-20 11:36:18.344
Last review date 2018-07-20 11:36:25.58

Subject Title
Protein-Based Technology 1

Subject Description
Overview of Protein-based  technologies; examples of applications in different biotechnology sectors; basic methods for protein extraction, quantification, isolation and purification; methods of extraction from tissues and micro-organisms; protein quantification using standard colorimetric assays and purification by chromatography,  affinity chromatography; protein analysis by denaturing polyacrylamide gel electrophoresis (SDS-PAGE); practical examples, theory and laboratory practices. (Lecture & Lab).

Credit Status
One credit towards the Applied BioChem Techniques (ABCT) Certificate Program (APPLIED BIOTECHNOLOGY).

Learning Outcomes
Upon successful completion of this subject the student will be able to:

  1. Describe protein based technologies – processes and protocols, pre -requisites
  2. Examine the details of the protein isolation, extraction and purification techniques
  3. Perform different types of chromatography – affinity, HPLC
  4. Learn applications of different protein based technology to real industry scenarios – forensic medicine, vitamin and antibiotic production
  5. Perform electrophoresis techniques in the Biotechnology laboratory

Cheating and Plagiarism
Each student should be aware of the College's policy regarding Cheating and Plagiarism. Seneca's Academic Policy will be strictly enforced.

To support academic honesty at Seneca College, all work submitted by students may be reviewed for authenticity and originality, utilizing software tools and third party services. Please visit the Academic Honesty site on for further information regarding cheating and plagiarism policies and procedures.

All students and employees have the right to study and work in an environment that is free from discrimination and/or harassment. Language or activities that defeat this objective violate the College Policy on Discrimination/Harassment and shall not be tolerated. Information and assistance are available from the Student Conduct Office at

Accommodation for Students with Disabilities
The College will provide reasonable accommodation to students with disabilities in order to promote academic success. If you require accommodation, contact the Counselling and Disabilities Services Office at ext. 22900 to initiate the process for documenting, assessing and implementing your individual accommodation needs.

Completion of BOT 100

Topic Outline
Week 1: test and assignment dates; assignment of topics for student’s presentations; introduction to protein based bio-techniques, examples and applications in different biotechnology sectors; Outline requirements for working in the laboratory, rules, expectations and safety. Laboratory: preparation of cultures of Gram + and Gram – bacteria for protein extraction.

Week 2: methods for protein extraction from microorganisms and tissues; different cell lysis techniques: colorimetric assays for protein quantification, commercially available kits and reagents. Laboratory: extraction and quantification of proteins from bacteria and fungi; quantification using colorimetric assays; preparation of SDS- polyacrylamide gels.

Week 3: non-denaturing and denaturing polyacrylamide gel electrophoresis (PAGE); one-dimensional and two-dimensional protein separation; principle and applications. Laboratory: separation and analysis of proteins from bacterial crude extracts; analysis of recombinant protein expression by SDS-PAGE.

Week 4: review of tissue culture techniques. Laboratory: extraction and analysis of proteins from cell cultures; methods for lysis of monolayer and suspension cultured mammalian cells; analysis of heat shock protein expression in several cell lines exposed to different temperatures by SDS-PAGE.

Week 5: review of laboratory results and midterm practical test.

Week 6: protein purification techniques; gel filtration, ion-exchange and high-pressure liquid chromatography (HPLC). Laboratory: separation and purification of heat shock proteins from crude cell extracts by gel filtration chromatography.

Weeks 7 and 8: review of immune staining techniques. Laboratory: detection of heat shock proteins (hsp) in crude cell extracts and gel filtration chromatographic fractions by Western-blot using commercially available anti-hsp antibodies.

Week 9: review of laboratory results and students’ presentations.

Week 10: Final theory and practical exam.

Mode of Instruction
Lectures, Laboratory, Tutorials, Assignment Presentations, Discussions

Prescribed Texts
To be prescribed by the instructor.

Reference Material
Prescribed text books, Laboratory experiment notes in form of procedures, technical manuals and reports

Required Supplies
Laboratory coat, safety glasses

Promotion Policy

Grading Policy
A+ 90%  to  100%
A 80%  to  89%
B+ 75%  to  79%
B 70%  to  74%
C+ 65%  to  69%
C 60%  to  64%
D+ 55%  to  59%
D 50%  to  54%
F 0%    to  49% (Not a Pass)
EXC Excellent
SAT Satisfactory
UNSAT Unsatisfactory

For further information, see a copy of the Academic Policy, available online ( or at Seneca's Registrar's Offices.

Modes of Evaluation

  • Assignments are due at the beginning of the class on which they are due.
  • A late penalty of 10% per day is assessed for late assignments, including those not handed in at the beginning of class when due.
  • Material will not be accepted after one week following the due date and/or when the marked material is returned to students, whichever comes first.
  • Assignments are to be prepared by computer.

Absenteeism and Exams/Quizzes
  • Students should be aware that absenteeism almost guarantees an inability to achieve satisfactory grades.
  • Students who are absent for an examination due to an emergency (e.g., motor vehicle accident, hospitalization or death in the family) may provide official documentation within five days of the missed exam and be provided a deferred exam at a later date.  Official documentation includes a death notice or an original doctor’s certificate identifying the date, length of time expected absence and the specific reason for the absence.  Examinations missed without official documentation and approval result in a grade of zero.
  • There are no deferred options for missed quizzes.  The value of missed quizzes, at the discretion of the Faculty, will be added to other evaluation components.

English Proficiency
  • All written work should demonstrate the following characteristics for clarity and conciseness:
-writing is consistent with the rules of English grammar
-spelling and punctuation are correct
-sentences are structured correctly
-main ideas are supported with specific, relevant examples and reasons
-work flows logically through supporting statements/paragraphs
-work is arranged in correct format (e.g., as a report, essay)
-up to 10% of the final grade may be deducted on all work if the above English competencies are not met.

Format for Assignments
  • Students must use the standard, APA style for quoting sources.   Help is available at:

Laboratory Attendance
The laboratory component is essential and therefore it is strongly recommended that you attend all labs.  Any missed labs must be supported with a legal document with three days of the lab.  Any student who fails to attend more than 2 laboratory classes for a 10 week subject will not pass the subject.    

Laboratory Safety
Students are required to review and understand the safety procedures and guidelines outlined on the first class and sign the sheet to this effect before beginning work in the laboratory.  Students must also wear a lab coat and safety glasses when conducting experiments.
A student who arrives without the proper safety equipment will not be permitted to participant in the lab but will be asked to leave the class.  The student will receive no grade for the lab missed.

Grading is based on the following marking scheme:
Laboratory reports 20%
Assignments 10%
Midterm test 30%
Final Exam  40%


Other Evaluation Considerations
The student is expected to comply with the Safety Rules for working in the laboratory, sign the safety contract, and WILL NOT UNDER ANY CIRCUMSTANCES WEAR CONTACT LENSES in the laboratory. The student will know where all safety equipment is located in the laboratory and will be familiar with WHIMIS concepts and signage.


Approved by: Denis Gravelle